5. Environmental proteomics

5b. The effects of sewage sludges were investigated on the symbiotic interactions between the model plant M. truncatula

and the AM fungus Glomus mosseae	or the rhizobial bacteria S. meliloti.

By comparison to a control sludge showing positive effects on plant growth and root symbioses, sludges enriched with polycylic aromatic hydrocarbons or heavy metals were deleterious.
Symbiosis-related proteins were detected and identified by 2-DE and MALDI-TOF MS, and image analysis was used to study the effects of sewage sludges on M. truncatula symbiotic proteome.

Impact of sewage sludges on the amount of symbiosis-related proteins Spot Putative functiona Spot volumes (%)b b2 b21 b22 y57 Glutathione transferase 60 a 22 b 24 b y65 Cytochrome c oxidase subunit 6b 119 a 43 b 50 b z12 Ascorbate peroxidase 61 a 22 b 0 b y22 Peroxidase 1A precursor 61 a 92 b 75 a y73 Polygalacturonase inhibitor protein 52 a 40 a 59 a y89 Profucosidase precursor 21 a 20 a 21 a z8 NAD(p)h dependent 60-deoxychalcone synthase 119 a 118 a 120 a y24 Elongation factor Tu 102 a 104 a 148 b y25 Enolase 142 a 139 a 196 b y28 Heat shock protein 70 104 a 103 a 144 b y44 NifK nitrogenase Fe–Mo beta chain 76 a 101 b 124 c z43 Transcription elongation factor 89 a 81 a 109 b z46 Nucleoside-diphosphate-kinase 78 a 108 b 129 c y76 Superoxide dismutase 95 a 111 b 117 b y93 Leghemoglobin 3 102 a 77 a 134 c z26 GTP binding protein 100 a 99 a 119 b z27 Elongation factor Tu 98 a 106 a 122 b z31 Leucine speci.c binding protein precursor 103 a 102 a 124 b z35 Amino acid binding periplasmic protein 91 a 74 a 121 c Lb1 Leghemoglobin 1 104 a 81 b 112 a y107 Leghemoglobin 2 121 a 118 a 105 a y75 Nitrogenase iron protein 122 a 97 b 145 a z40 30S ribosomal protein S2 102 a 111 a 105 a y46 Malate dehydrogenase 82 a 99 b 84 a a Identifications were realized in this work or in Bestel-Corre et al. (2002). b Spot volumes in B0 treated plants were normalized to 100% and the volumes of corresponding spots in B2, B21 and B22 treated plants were calculated accordingly. Datas are the means of three replicates. For each spot, numbers with the same letter do not differ significantly.