Proteomics for studying arbuscular mycorrhizal symbioses

Proteomics represents a powerful tool for studying proteins and their post-translational modifications under different developmental stages or in response to various environmental stimuli. In particular, it appears especially well dedicated to the study of the arbuscular mycorrhizal (AM) symbiosis [1, 2].
As a complement to EST and gene expression studies, we are carrying out proteome analyses of Medicago truncatula roots during their colonisation with arbuscular mycorrhizal fungi. For this purpose, several complementary strategies are being developed :

1. Comparative proteomic analysis of M. truncatula Jemalong J5 roots in interaction either with an AM fungus or a bacterium

2. Sub-cellular root membrane proteomics of AM symbiosis

2a. Reference map of microsomal root proteins
2b. Comparative extrinsic membrane proteomics of the AM symbiosis
2c. Plasma membrane root proteomics

3. Comparative proteomics of the early stages of the AM symbiosis

3a. Protocol for analysing mRNAs and proteins at the same time
3b. Comparative analyses

4. AM fungal proteomics

5. Environmental proteomics

5a. We used a targeted proteomics to identify cadmium-induced protein modifications in Glomus mosseae-inoculated pea roots
5b. The effects of sewage sludges were investigated on the symbiotic interactions between the model plant M. truncatula

6. Collaborations