Comparative proteomic analysis of M. truncatula Jemalong J5 roots in interaction either with an AM fungus or a bacterium

1. The comparative proteomic analysis of M. truncatula Jemalong J5 roots in interaction either with of the arbuscular mycorrhizal fungus Glomus mosseae (Nicol. & Gerd.) Gerd. & Trappe (BEG 12) or the bacterium Sinorhizobium meliloti (RCR 2011) has been carried out.

Differentially displayed proteins during mycorrhization and nodulation were detected by two-dimensional electrophoresis (2-DE) during time course experiments going from 2 to 42 days post inoculation, some of which were identified by MALDI-TOF or tandem mass spectrometry [3, 4].

RESULTS 2 DE pattern of M. truncatula 3 week old control roots. One hundred microgram proteins were separated onto first dimension pH 3-10 IPG gels and second dimension 12% vertical slab gels. Click on areas A to F to view detailed pictures.

Identified proteins Control G. mosseae - inoculated S. meliloti - inoculated

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