mt proteomcis, symbiotic proteomics of Medicago truncatula

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4. AM fungal proteomics

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To identify fungal proteins involved in the AM symbiosis, root-inducing transferred-DNA transformed roots of carrot (Daucus carota L.) were in vitro inoculated with Glomus intraradices. Proteins extracted from the extraradical fungus were analyzed by 2-DE. A fungal reference map displaying 438 spots was set up.
Four proteins, among the fourteen selected for tandem mass spectrometry analysis, were identified. This is the first report of AM fungal protein identifications by using a proteomic approach.

 


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